2007
J.D. Ng, R.C. Stevens,
P. Kuhn “Protein crystallization
in restricted geometry: advancing old ideas for modern times in structural
proteomics” in Methods in
Molecular Biology. B. Kobe, M. Guss and T. Huber, Eds., in press, (2007).
E. Abola, D.D. Carlton,
P. Kuhn, R.C. Stevens “Five years
of increasing structural biology throughput. A retrospective analysis”
in Structure Based Drug Discovery,
Chapter 1, H. Jhoti and A. Leach, Eds., in press (2007).
Q. Zhang, X. Ma, A. Ward, W.X. Hong, V.P. Jaakola, R.C. Stevens, M.G. Finn, and G. Chang “Designing facial amphiphiles
for the stabilization of integral membrane proteins” Angew. Chem. Int. Ed. Engl. In press, (2007). DOI:10.1002/anie.200701556
M.A. Hanson, A. Brooun, K.A. Baker,
V.-P. Jaakola,
C. Roth, E. Chien, A. Alexandrov, J. Velasquez, L.
Davis, M. Griffith, K. Moy, B. Ganser-Pornillos,
P. Kuhn, S. Ellis, M. Yeager, and R.C. Stevens “Profiling of membrane protein variants in a baculovirus
system by coupling cell-surface detection with small-scale parallel expression”
Protein Expr. Purific. In press (2007). DOI:10.1016/j.pep.2007.06.003
2006
C.J. Gerdts, V.
Tereshko, M.K. Yadav, I. Dementieva, F. Collart, A. Joachimiak, R.C. Stevens,
P. Kuhn, A. Kossiakoff, R.F. Ismagilov “Time-controlled microfluidic seeding in nL-volume droplets to separate
nucleation and growth stages of protein crystallization.” Angew. Chem. Int. Ed. Engl. 45, 8156-8160 (2006). DOI:10.1002/anie.200602946
R.C. Stevens “SPINE Forward” Acta Crystallogr. D62, 1 (2006).
E. Abola, P. Kuhn, R.C.
Stevens “Miniaturization of the
structural biology technologies – From expression to biophysical analyses”
in Structural Genomics on Membrane
Proteins. K. Lundstrom, Ed., CRC Press: Boca Raton, FL. Pp. 299-320,
(2006).
2005
M.K. Yadav, C.J. Gerdts, R. Sanishvili, W.W. Smith,
L.S. Roach, R.F. Ismagilov, P. Kuhn, R.C. Stevens “In situ data collection and
structure refinement from microcapillary protein
crystallization” J. Appl. Crystallogr. 38, 900-905 (2005).
DOI:10.1107/S002188980502649X
B. Collins, R.C. Stevens, R. Page “Crystallization optimum solubility
screening: Using crystallization results to identify the optimal buffer for
protein crystal formation” Acta Crystallogr. F61, 1035-1038 (2005). PMID:16511228
W. Peti, R. Page, K. Moy, M. O’Neil-Johnson, I.A. Wilson, R.C. Stevens, K.
Wüthrich “Towards
miniaturization of a structural genomics pipeline using micro-expression and microcoil NMR” J. Struct. Funct. Genomics, on-line November 9, 2005. (2005).
PMID:16283429
R. Page, A.M. Deacon, S.A. Lesley, R. C. Stevens
“Shotgun crystallization strategy
for structural genomics II:
Crystallization conditions that produce high resolution structures for T. maritima proteins” J. Funct. Struct. Genomics, 6, 209-217 (2005). PMID:16211521
R. Page,
D.C. Carter, P. Rhodes, D.E. McRee, L. Tari, D.R. Dougan, G. Snell, E.
Abola, R.C. Stevens “Reduction in
diffuse-convective disturbances in nanovolume protein
crystallization experiments” J.
Appl. Crystallogr., 38, 87-90 (2005). DOI:10.1107/S0021889804028055
2004
J.M. Canaves, R. Page, R.C. Stevens "Protein biophysical properties that
correlate with crystallization success in Thermotoga Maritima: Maximum clustering strategy for structural genomics"
J. Mol. Biol., 344, 977-991 (2004). PMID:15544807
R. Page, R.C. Stevens "Crystallization data mining in structural genomics: using positive and
negative results to optimize protein crystallization screens" Methods, 34, 373-389 (2004). PMID:15325655
R. Page, K. Moy, E.C. Sims,
J. Velasquez, B. McManus, C. Grittini, T.L. Clayton, R.C. Stevens “Scalable high-throughput
micro-expression device for recombinant proteins” BioTechniques, 37, 364-370 (2004) PMID:15470889
M. Bern, D. Goldberg, R.C. Stevens, P. Kuhn “Automatic classification of protein
crystallization images using a curve tracking algorithm” J. Appl. Crystallogr., 37,
279-287 (2004). DOI:10.1107/S0021889804001761
G. Snell, C. Cork, R. Nordmeyer, E. Cornell, G. Meigs, D. Yegian, J. Jaklevic, J. Jin, R.C. Stevens, T. Earnest “Automated sample mounting and
alignment system for biological crystallography at a synchrotron source” Structure, 12, 537-545 (2004). PMID:15062077
R.C. Stevens. “Long live structural biology” Nature Struct. Mol. Biol., 11, 293-295 (2004). PMID:15048098
D. Pantazatos, J.S. Kim,
H.E. Klock, R.C. Stevens, I.A. Wilson, S.A. Lesley,
V.L. Woods Jr “Raid refinement of
crystallographic protein construct definition employing enhanced
hydrogen/deuterium exchange MS” Proc.
Natl. Acad. Aci U.S.A., 101, 757-762 (2004). PMID:14715906
2003
R.C. Stevens "The cost and value of three-dimensional protein
structure" Drug
Discovery World, 4, 35-48 (2003). [PDF]
R. Page, S.K. Grzechnik,
J.M. Canaves, G. Spraggon, A. Kreusch, P. Kuhn, R.C.
Stevens, S.A. Lesley "Shotgun crystallization strategy for structural
genomics: An optimized two-tiered crystallization screen against the Thermatoga maritima proteome" Acta Crystallogr. D59, 1028-1037, (2003).
PMID:12777766
D. Hosfield, J. Palan, M. Hilgers, D. Scheibe, D. McRee, R.C. Stevens "A fully integrated
protein crystallization platform for small molecule drug discovery" J.
Struct. Biol. 142,
207-217, (2003). PMID:12718932
M. Weselak, M.G. Patch,
T.L. Selby, G. Knebel, R.C. Stevens "Robotics
for automated crystal formation and analysis" in Methods in Enzymology. C.W. Carter & R.M. Sweet
(Eds.), Academic Press:
2002
P. Kuhn, K. Wilson, M.G. Patch, R.C. Stevens "The
genesis of high-throughput structure-based drug discovery using protein
crystallography" Curr. Opin.
Chem. Biol., 6, 704-710 (2002).
PMID:12413557
S.A. Lesley, et al. "Structural genomics of
the Thermotoga maritima proteome
implemented in a high-throughput structure determination pipeline" Proc.
Natl. Acad. Sci. USA, 99, 11664-11669
(2002). PMID:12193646
T.L. Selby, R.C. Stevens "Bioinformatics and
high-throughput protein production for structural genomics" in Gene
Cloning and Expression Technologies, M.P. Weiner & Q. Lu (Eds)., Biotechniques Press:
B.D. Santarsiero, D.T. Yegian, C.C. Lee, G. Spraggon, J. Gu,
D. Scheibe, D.C. Uber, E.W.
Cornell, R.A. Nordmeyer, W.F. Kolbe, J. Jin, A.L. Jones, J.M. Jaklevic, P. G. Schultz, R.C. Stevens "An approach
to rapid protein crystallization using nanodroplets"
J. Appl. Crystallogr.,
35, 278-281 (2002). DOI:10.1107/S0021889802001474
2001
R.C. Stevens, S. Yokoyama, I.A.
Wilson “Global efforts in structural genomics” Science,
294, 89-92 (2001). PMID:11588249
R.C. Stevens, I.A. Wilson “Industrializing
structural biology“
Science, 293, 519-520 (2001). PMID:11463918
K.E. Goodwill, M.G. Tennant, R.C. Stevens “High-throughput
X-ray crystallography for structure-based drug design“ Drug
Discovery Today, 6, S113-8 (2001).
DOI:10.1016/S1359-6446(01)00169-6
2000
H. Erlandsen, E. Abola, R.C. Stevens "Combining
structural genomics and enzymology: Completing the
picture in metabolic pathways and enzyme active sites" Curr. Opin. Struct. Biol., 10,
719-730 (2000). PMID:11114510
E. Abola, P. Kuhn, T. Earnest, R.C. Stevens "Automation
of X-ray crystallography" Nature Struct. Biol., 7, 973-977 (2000). PMID:11104004
R.C. Stevens "High-throughput
protein crystallization" Curr. Opin. Struct. Biol., 10,
558-563 (2000). PMID:11042454
R.C. Stevens "Design of high-throughput
methods of protein production for structural biology" Structure
Folding and Design, 8, R177-R185, (2000). PMID:10986469
last updated September 7, 2007 - send corrections and comments to Angela Walker (alwalker@scripps.edu)